Antioxidant Activity of Metanol Extract of Sea Weeds Obtained from North Sulawesi

Abstract

In Vitro antioxidant activity of four sea weeds from North Sulawesi –viz., Gracillaria salicornia, Sargasum olygocystum, Turbinaria decurens,  Halimeda macroloba and H. durvilae were evaluated. Total phenol content (TPC) and activity antioxidant of 30%,50%, 70% aqueous  were study. TPC  using follin Ciocalteu method,  while  1,1-diphenyl-2pycrylhydrazyl (DPPH) radical scavenging, Ferric reducing Antioxidant Power (FRAP) and ferrous ion Chelating (FIC) assay  were used to study their AOC(Antioxidant Capacity) . The total phenol content of different metanol concentration of sea weed varied from 3.02±0.68% to 18.83±0.77 g gallic acid equivalent (GAE) per 100 gr dried sample, All sea weed  were determined to have highest TPC, DPPH and FRAP  in  70 % aqueous metanol . H durvilae displayed highest in TPC and DPPH. G.salicornia showed highst in FRAP. 42.95±2.24 g GAE/100 g ekstrak. Brown alga T. decurens and S. olygocystum have FIC activity higher then another seaweeds in 30% metanol consentration.

Keywords: Antioxidant Activity, Metanol Extract , Sea Weeds