A New Isocratic HPLC Method for Simultaneous Determination of Paracetamol and Flurbiprofen in Pharmaceutical Preparations

Neset Nesetoglu, Serap Saglik Aslan, Durisehvar Ozer Unal


An isocratic HPLC method has been developed for simultaneous determination of paracetamol-acetaminophen (PAR) and flurbiprofen (FLU) in pharmaceutical preparations. The method was based on separation by using a mobile plase acetonitrile– 0.1M sodium acetate solution (pH:5.00) (60:40,v/v) and reversed-phase C18 column. The mobil plase flow rate was 0.8mL/min with UV detection at 247nm. The linearity ranges were 0.1-1.5 μg/mL for PAR and FLU. The limits of detection and quantification were found to be 16.33 ng/mL and 54.45 ng/mL for PAR and 15.25 ng/mL and 50.82 ng/mL for FLU, respectively. The combination of these two drugs has not commercially avaible but patented as combined preparation. The proposed method was successfully validated and applied to the determination in pharmaceutical preparations. The developed and validated method is simple, sensitive and reproducible and can be used safely routine simultaneous analysis of PAR and FLU in pharmaceutical preparations in the future.

Keywords: Paracetamol; Acetaminophe; Flurbiprofen; HPLC; Pharmaceutical preparations.

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ISSN (online) 2422-8702