Biochemical and Histopathological Markers as Indicators of Genuine Toxicity Caused by Intraperitoneal Application of Tilt 250 Fungicides of Fish Barbus Peloponnesius

Abstract

In this paper are presented the obtained results from the research activity of the enzyme so-called Ethoxyresorufin-O-deethylase in the liver of the fish Barbus peloponnesius (Valenciennes, 1842), after intraperitoneal application of certain doses of Tilt 250 fungicide (0.18mg/l, 0.36mg/l and 1.08 mg/l). Also in this paper are presented the results of histopathological analysis of liver, kidney and gonads after the intraperitoneal application of certain dose Tilt 250 fungicide (2?g/l), during the 1,2,3,7 and 14 days. From the obtained results it was concluded that the application of the doses of 0.18mg/l, 0.36mg/l and 1.08mg/l to 250 tilt fungicides have caused the induction of EROD enzyme, whereas the dosage application of 2?g/l toxicity has caused changes in the liver, kidney and gonads.

Keywords: pesticides, fish, biomarker, EROD, lesions.