Role of Isoflavones on Lipid Peroxidation, Superoxide Dismutase in Lymphocytes under Oxidative Stress Conditions

Abstract

Oxidative stress conditions forms free radicals in cells. Free radicals react continuously and cause cell membranes damage including lymphocytes, thus decreasing cell function. Therefore, there were efforts need to be done to overcome abnormalities and decreased lymphocyte cell function under conditions of oxidative stress. One of them using bioactive compounds that have antioxidant activity, such as isoflavones. Isoflavones have been reported to have antioxidant activity and the potential to protect cells of oxidative stress. This study aims to determine the role of isoflavones as antioxidative in protecting lymphocytes under conditions of oxidative stress, be reviewed of CD4, CD8, malondialdehyde levels and superoxide dismutase activity. This study was used rat lymphocyte cell cultures in two conditions, i.e: oxidative stress and without oxidative stress. The cell culture were treated with 20 ml of 2 mM paraquat and  isoflavone at dose 0, 1000, 2000, and 3000 ppm, respectively. The parameters were observed: (1) malondialdehyde  (MDA) levels,  (2) superoxide dismutase (SOD) activity, and (3) percentage of CD4 and CD8 lymphocytes cell using a monoclonal anti-rat CD4 and CD8 FITC-conjugate. The results showed paraquat administration in cell culture on oxidative stress conditions could reduce the number of CD4 and CD8 lymphocytes cels, increased of malondialdehyde (MDA) and superoxide dismutase (SOD) activity. Administration of isoflavones on culture that treated without oxidative stress condition could increase percentage number of CD4, CD8, MDA levels, and SOD activity. Whereas, administration of isoflavones on oxidative stress condition prevented the decrease percentage number of CD4, CD8, MDA levels, and SOD activity. Administration of isoflavone at dose 3000 ppm in cell culture that treated on oxidative stress condition provided optimal results in preventing the decrease number of CD4, CD8 lymphocytes cell, MDA levels, and SOD activity.

Keywords: isoflavone, lymphocytes, CD4, CD8, malondialdehyde, superoxide dismutase.