Chromatographic Separation of Deuterium-Labeled Amino Acids, Proteins, Carbohydrates and Lipids Isolated From Bacterial Objects

Oleg Mosin, Ignat Ignatov


We carried out the chromatographic separation of 2H-labeled amino acids, proteins, carbohydrates and lipids isolated from biological samples of various taxonomic groups of prokaryotic and eucaryotic microorganisms realizing methylotrophic, chemoheterotrophic, photo-organotrophic, and photosynthetic pathways of assimilation of carbon substrates (methylotrophic bacteria Brevibacterium methylicum, chemoheterotrophic bacteria Bacillus subtilis, photo-organotrophic halobacteria Halobacterium halobium, green algae Chlorella vulgaris) at the growth on media with heavy water (2H2O). For all investigated microorganisms are submitted data on growth and adaptation on growth media containing as sources of deuterated substrates 2H2O, [2H]methanol and hydrolysates of deutero-biomass of methylotrophic bacteria B. methylicum, obtained after the multistage adaptation to 2H2O. The qualitative and quantitative composition of intra- and endocellular 2H-labeled amino acids, proteins, carbohydrates and lipids in conditions of adaptation to 2H2O is investigated. The chromatographic separation was implemented by the methods of RP-HPLC, ion exchange chromatography and electrophoresis.

Key words: amino acids, bacteria, carboxydrates, lipids, heavy water

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