Effective HPLC method development

Abstract

High Pressure Liquid Chromatography (HPLC) method development plays an important role in the discovery, development and manufacture of pharmaceutical products and day to day quality work in the laboratory. This article focuses on the effective method development of HPLC. It highlights pertinent conditions and other important perspectives during method development.  A sequence of events required for effective method development is described. The steps involved in developing a stability-indicating HPLC method influences the analysis of degradation products/impurities in stability study and its validation demonstrate the suitability for its intended purpose.

Keywords: Method development, High Pressure Liquid Chromatography (HPLC), analytes.