Effect of Fermented Lagenaria (Adenopus breviflorus) Fruit Extract on the Heamatological and Serum Biochemical Indices of Broiler Chickens

Abstract

The experiment involved 126 day old broiler chicks (Arbor acre plus) which lasted for six weeks after two weeks of acclimatization. The project was carried out in a completely randomized block design to evaluate the haematological and serum biochemical parameters of broilers served fermented lagenaria fruit extract (FLFE) at three days interval. The birds were weighed and randomly distributed into six dietary treatment group. Birds in treatment A (control) were given vaccine and drugs only, birds in treatment B were given vaccine only, birds in treatment C were given drugs only, but birds in treatments D, E and F were served (100, 200 and 300)ml of FLFE in 250ml of water, respectively. Each treatment was replicated three times with seven birds per replicate. The birds were maintained on starter and finisher marsh for starter and finisher phase, respectively. Feeds and water were served ad libitum. Data collected were subjected to Analysis of Variance (ANOVA) and comparisons were made using Duncan’s Multiple Range Test and significance was accepted at (P<0.05). The parameters tested were packed cell volume (PCV), haemoglobin (Hb), red blood cell (RBC), white blood cell (WBC), platelet (P), mean cell volume (MCV), mean cell haemoglobin (MCH), mean cell haemoglobin concentration(MCHC), lymphocyte (LYM), heterocytes (HET), monocytes (Mn), eosinophils for haematological indices and total serum protein (TSP), albumin(Al), globumin (Gb), creatinine (Cr), alanine amino transminase (ALT), aspatate amino transminase (AST), alkaline phosphate (ALP), blood urea nitrogen (BUN) and cholesterol (CH) for serum biochemistry. The results showed significant (P<0.05) effects of the parameters studied across the treatment groups. The PCV was highest (40.00%) on the birds placed in control, and least on the birds served 100-300ml FLFE (29.50-33.00%). Similar scenario was observed for the birds in control for the Hb (13.20%) compared to those served 100-300ml FLFE (9.40-10.70%). The birds in treatments C (drugs only), D (100ml FLFE) and those in F (300ml FLFE) had the highest concentration of white blood cells which were 1.92, 2.12 and 1.87x10⁴/ml, respectively. The birds served 100-300ml FLFE had reduced concentration of Hb of 21.25% compared to control, and they had elevated concentration of WBC of 25.82% compared to control. The birds placed on vaccines only and drugs only had elevated WBC of 26.00 and 34.50%, respectively compared to control. The FLFE of 100-200ml had elevated platelet (353.00-314.50x10³/ml) with reference to control. The FLFE had no significant (P<0.05) effect on TSP, GB, AL, ALP and BUN. However, concentration of CH increased with increased concentration of FLFE as the bird offered 100ml had CH of 42.50mg/dl, compared to those on 200ml and 300ml whose value were 68.50 and 89.00mg/dl, respectively. Broiler chicken can tolerate 100-300ml FLFE for improved blood formation.

Keywords: Lagenaria, Hematology, Serum biochemistry, Broiler, Vaccine