Isolation, Identification and Characterization of Urinary Tract Infectious Bacteria and the Effect of Different Antibiotics

Geoffrey Arasa Ouno, Scolastica Chepngetich Korir, Joan C. Cheruiyot, Ongechi Donald Ratemo, Benard Maronga Mabeya, Godfrey Omare Mauti, Eliakim Mbaka Mauti, Sabella J. Kiprono


Introduction: Urinary Tract Infection (UTI) defines a condition in which the urinary tract is infected with a pathogen causing inflammation which is a common, distressing and occasionally life threatening condition. UTI affects people of all  ages and both gender. In all patients with UTI are reported with asymptomatic bacteriuria. Female are more susceptible to  UTIs compared to  male. To ensure appropriate therapy, current knowledge of  the  organisms  that  cause  UTI  and  their  antibiotic  is  susceptibility is mandatory.

Methods: This study focused on the frequency of uropathogens and  their antibiotic

susceptibility in different gender in Madurai District. Cultural and biochemical characterization  of  uropathogens revealed  the  prevalence  of  both  gram-positive and gram-negative organisms

Results: E. coli was the predominant isolate isolated from the urine specimen followed by Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Proteus mirabilis and Enterococcus faecalis. Among the antibiotics tested, chloraphenicol and ciprofloxacin (100%) were found to be effective for empirical treatment of UTI and has covered the majority of urinary pathogens followed by tetracycline,  gentamycin and kanamycin (83%), Ampicillin (67). Streptomycin, Rifampicin and amoxicillin were less effective (50%).

Conclusion: Some of the isolates were resistant to penicillin-G, Streptomycin, rifampicin and amoxicillin which are more frequently prescribed and indicates that increased consumption of a particular antibiotic leads to acquisition of resistance by the uropathogens.   Resistance rates among common uropathogens continue to evolve and appear to be increasing too many commonly used antimicrobial agents and a continued surveillance of resistance rates among uropathogens is needed to ensure appropriate recommendations for the treatment of the urinary tract infections.

Keywords: Urinary tract infection, Drug resistence, Uropathogens, Biochemical tests.

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