The Activities of Pumelo Fruit Juice (Citrus maxima var nambangan), Vitamin C and Lycopene against Hepatotoxicity Ochratoxin Exposure Prevention on Induced Mice (Mus musculus)

Abstract

The aim of this study was to know the potency of pumelo fruit juice (Citrus maxima var nambangan), vitamin C, lycopene, and combination of vitamin C and lycopene, as antioxydant compound on hepatotoxicity of ochratoxin A (OTA) on induced mice (Mus musculus).

Thirty-five male mice (Mus musculus) strain of balb/c, with age between two and three months were divided randomly into seven groups of treatments (n=5), including K0, Kl, K2, P1, P2, P3, P4. Each from respectively group for control group was feed only with Olive Oil adjuvant (KO), CMCNa (K1), ochratoxin A (K2), pumelo fruit juice (citrus maxima var nambangan) dose of 0,5 ml/30 gram BW/day (PI), vitamin C dose of 5,85 µg (P2), lycopene dose of 0,1025 µg (P3), and the combination of vitamin C dose of 5,85 µg /30 g BW mice and lycopene dose of 0,1025 µg/30 g BW (P4). The experiment was conducted for three weeks periode, which is K0, Kl, K2 groups were subjected with compound of treatment for a week period started at second week, whereas P1, P2, P3, and P4 groups were subjected with compound of antioxidant treatment for two weeks period started on second week, and subjected with ochratoxin A dose 1 mg/kg BW/day for a week in the beginning of the third week. All the experimentation mice were sacrifice for data collection on the 21^st of days.

The statistical analisis result was analyzed with Kruskal Wallis test which continued with Mann-White showed there was a significant different (p<0.05) between groups of treatment. It has been proved that all groups subjected to antioxidant (PI, P2, P3, dan P4) significantly (p<0,05) were able to prevent the damage of liver cell after the ochratoxin A exposure, which was indicate on the descending of necrotic liver cell.

This research also proved there was no significant different (p>0.05) the biologic potency of Pummelo fruit juice compare with vitamin C, lycopene and the combination of lycopene and vitamin C on inhibited free radical reactivity due to OTA exposure on mice’ liver.

Keywords: Pumelo Fruit (Citrus maxima var nambangan), Ochratoxin, Hepatotoxicity