Supplementation with Goat Follicular Fluid in the In Vitro Maturation Medium toward Cumulus Expansion and Nucleus Transformation

Nolasco Da Costa, Sri Wahjuningsih, Nurul Isnaini


The purpose of the research was to the influence of goat follicular fluid (GFF) with different levels in maturation medium in vitro on cumulus expansion and  transformation of goat oocyte nucleus. The results of this study  showed that cumulus cell expansion was related to the higher level of different GFF in maturation medium TCM-199. Cumulus cell expansion, and increased nucleus transformation occurs at level of 10% and decreased  medium without GFF. This suggests that cumulus expansion and nucleus transformation decreases, because the need of oocyte is not supported by hormones and nutrients in the maturation medium.

Key words: GFF, TCM-199, cumulus expansion and maturation of the nucleus

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ISSN (Paper)2224-3208 ISSN (Online)2225-093X

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