Combined Methanolic Leaf Extracts of Vernonia amygdalina and Gongronema latifolium Improves Sperm Parameter Impairment and Testicular Damage in STZ Induced Diabetic Wistar Rats

Akpaso, M. I, Igiri, A. O., Fischer, V. A, Fischer, C. E, Asuquo, O.R


The effects of  methanolic leaf extracts of Vernonia amygdalina (VA) and Gongronema latifolium (GL) on the histology and DNA of the testes, and the sperm parameters of streptozocin (STZ) induced diabetic rats were investigated in this study. 18 male albino rats which were divided into three groups of six rats each were used for this work: A (Normal control) and B (Diabetic control) received tap water, C received combined extract of VA and GL, (400mg/kg twice daily, 200mg/kg each extract). Groups B &C animals were induced for diabetes by intraperitoneal injection of 45mg/kg of Streptozocin, reconstituted in 0.1M sodium citrate buffer. Following sacrifice, the testes and semen were collected for histopathological studies and semen analysis respectively. The results revealed normal testes with prominent seminiferous tubules, containing germ cells at various stages of development and spermatozoa in group A. In group B, there were signs of degeneration in the seminiferous tubules (STs), destruction of germ cells, spermatozoa and Leydig cells. In group C, the testes showed normal STs with Sertoli cells, Leydig cells, germ cells and spermatozoa, suggestive of a possible regeneration. Feulgen’s reaction showed damage to the DNA of the testes in group B which was almost completely reversed in group C. Semen analysis revealed reduced sperm count, high percentage of abnormal forms and high percentage of spermatozoa with retarded motility in group B compared to group A. In group C, there was marked improvement in all the parameters.

Keywords: Diabetes mellitus, sperm parameters, testicular damage, Vernonia amygdalina, Gongronema latifolium

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