Effects of the Aqueous Root Extract of Vernonia amygdalina on the Haematological Profile of Rattus norvegicus

Abstract

The effects of Vernonia amygdalina root extract on the haematological profile of 20 male albino rats (Wistar strain) were investigated for a period of 21 days. The rats were divided into three treatment groups A, B, and C and a control group D. The experimental groups A, B, and C were treated with 50mg.Kg^-1, 150mg.Kg^-1, and 300mg.Kg^-1 body weight respectively while the control (group D) received equal volume of normal saline. The extracts were given to the animals orally for 21 days. Blood was collected through the orbital-plexus of rats to assay the effect of the extract on packed cell volume (PCV), white blood cell count (WBC), red blood cell count (RBC) and haemoglobin (Hb) level. The mean WBC, RBC, PVC and haemoglobin level ranged from 1560 ± 120 to 3200 ± 1200, 150.00 ± 40.00 to 276.00 ± 44.50, 38.00 ± 1.00 to 41.00 ± 3.00, and 16.00 ± 2.00 to 12.50 ± 0.50, respectively. There was no significant different (p>0.05) in the haematological profile of the treated groups when compared with the control. However, there was significant different (p<0.05) within each group from week one to week three except WBC in group D and PVC in all the groups. This suggested that the effect of the aqueous extract of V. amygdalina on the haematological profile of Rattus norvegicus was dependent on duration of consumption.

Keywords: Vernonia amygdalina, Aqueous root extract, Haematological profile, Rattus norvegicus