Isolation and Assessment of Antagonistic Microbial Interactions from Environmental Samples

Abstract

This study assessed the intricate patterns of antagonistic microbial interactions in the quest to help manage and promote human and animal health, and contribute to our understanding of complex microbial communities, like the human gut microbiome. Antagonistic microorganisms are known to possess different kinds of metabolites, antibiotics, etc., that can inhibit the growth of pathogens around them. The identification of antagonistic microorganisms from environmental samples holds significant potential for application in areas like agriculture, biotechnology, environmental bioremediation, and even healthcare. This study focused on the isolation of microbial species that exhibit antagonistic interactions, with a specific emphasis on their inhibitory effects on pathogenic organisms. Environmental samples were collected from environmental sources, particularly from air and soil sources, and subjected to serial dilution techniques. The resulting microbial isolates were cultured on selective media to foster antagonistic interactions. Various microbial strains were successfully isolated and identified. Through analyses, antagonistic interactions were observed among bacterial-fungal pairs. The findings reveal a rich diversity of antagonistic microorganisms, with potential applications ranging from agriculture, mining, and biopharmaceutical production.

Keywords: Antagonism, Antibiotics, Antimicrobial Interactions, Bacteriocins, Pathogens, Bioremediation, Microbial Strains.

DOI: 10.7176/ALST/101-07

Publication date: December 31st 2025