Toxic Effects of Low Concentration of Cyanotoxin (Microcystin¬-LR) on Mice and Study of Protective Efficacy of the Antioxidants Vitamins (C&E) and Capparis spinosa L. Root Extract

Abstract

In this study the protective efficacy of vitamin C&E and  ethanolic  extract roots of C.spinosa against toxic effects of algal toxin (Microcystin –LR) in mice  were done .Results showed significant decreasing p≤0.05 in values of hemoglobin (HB) and packed cell volume  (PCV) reach to 7.77 and 27.23 g/l in blood of mice treated  with  low  concentration of cyanotoxin (MC-LR = 0.15 ug/kg/day) compared with control group which reach to 12.21 and  40.22 respectively. Significant increasing were showed in some liver enzymes  ALT,AST and ALP when exposed to toxin only  which reach to 68.80 , 167.85 and 274 U/l respectively compared with control group , While groups treated with vitamins and extract showed non -significant differences with control group in values of blood parameters and enzymes. Histopathological  changes in liver  when group treated with toxin only represented by pyknotic hepatocyte , hypertrophy , binucleation  , infiltration of lymphocyte, karyolysis. While  kidney showed hypertrophy of cell glomerular arterioles , metaplasia of squamous epithelial tissue of bowman capsule wall in to cuboidal, hypertrophy of renal tubules cells , hyperplasia , also results showed the important role of vitamin C&E and root extract of C. spinosa to keep the normal values of blood parameters and prevent histological changes as control group.

Keywords: Algal toxin ( Microcystin-LR) ,  protective efficacy of  Vitamin C&E  ,  C.spinosa