The Effect of NADH(H+) in the Oxidization of Benzidine in the H2O2 / Horseradish Peroxidase System

Abstract

The aromatic amines metabolized under the effects the enzymes  by different path way and  the peroxidase path is one of these methods, in Which aromatic amines changed from non-active into active form, Which can interact with the larges biological molecules as nucleic acids DNA and inhibited it . Intra cellular components performed multi-functional such as Glutathione G-SH , NADH(H⁺) and  NADP. It was chosen because of the high damper NADH concentration in the cell (10-3M)) and its spread in all types of tissues.NADH(H⁺) inhibit large number of products of oxidation reactions of the amino compounds enzymatically carcinogens, such as substrate benzidine and its derivatives such as 3,3',5,5' tetra methyl benzidine  in  Competitive path way.NADH(H⁺): Nicotinamide adenine dinucleotide( reduced form)

Keywords: Benzidine, , Peroxidase, inhibition , Nicotinamide adenine dinucleotide