Oxidative Stress Investigation in Anemia

Abstract

Introduction: Increased membrane rigidity, decreased deformability and hemolysis in erythrocytes are the result of oxidative damage. Our study included patients diagnosed with anemia and a control group of healthy individuals and in these groups, it was aimed to measure the level of Nitric Oxide (NO), which is an indicator of oxidative damage, and to determine the superoxide dismutase enzyme (SOD) activity, which are the most important components of the antioxidant system. Material and Method: Ethics Committee approval was obtained for the study and patients who were diagnosed with iron deficiency anemia, B12 deficiency anemia, Talassemia minor at the hematology polyclinic of the Manisa Celal Bayar University Medical Faculty Hafsa Sultan Hospital were included in the study. Nitrite / nitrate levels were measured spectrophotometrically, SOD enzyme activity was measured by ELISA method. Results: The difference between the study groups in terms of SOD values was not statistically significant. When nitric oxide results are compared; Thalassemia minor group values were significantly higher than control group (p <0.05). Conclusions: Increased levels of NO, especially in the thalassemia patient group, it is thought to be the cause of hemolytic anemia as a result of oxidative stress and lipid peroxidation in this patient group. Supporting Institutions: Manisa Celal Bayar University Scientific Researches Unit (2015-092).

Keywords: Anemia, Antioxidant System, Nitric Oxide, Superoxide Dismutase.

DOI: 10.7176/JHMN/75-03