Determination the Pathogenicity of C.difficile Isolates among Mice

Abstract

Clostridium difficile is the main causative agent of antibiotic-associated diarrhea and colitis in hospitalized patients, the disease is caused mainly by two exotoxins, TcdA and TcdB, produced by the bacteria. Recurrent C. difficile infection (CDI) constitutes one of the most significant clinical issues of this disease . This study aimed to Determination the pathological effects of C. difficile in mice, the results shown  that bacterial dose 1x10⁶ CFU\ml led to 50% death within (2-4) days post infection and 100% developed diarrhea ,that would cause infection but not universal lethality . In conclusion, we have established a mouse  CDI model that allows for future investigations of the role of the host immuneresponse in the disease’s pathogenesis and permits critical testing of new therapeutics targeting recurrent disease.

Keywords: C.difficile, dose, pathological  effects, mouse.