FREQUENCY OF VARIOUS RISK FACTORS FOR MECONIUM ASPIRATION SYNDROME

Objective: To study the frequency of various factor leading to meconium aspiration syndrome. Study Design : Descriptive / Cross sectional study. Place and Duration of Study : This study was conducted at the Department of Paediatrics Medicine Sir Ganga Ram hospital lahore from May _2016 to sep 2017. Materials and Methods: The non-probability, consecutive sampling technique was used. New-borns having staining of vocal cords and respiratory distress were included in this study. ABG and X-ray Chest was done in all neonates. Factors like, Post-term pregnancy, IUGR, thick meconium, low APGAR score at 1 and 5 minutes were noted. All the collected data was entered and analysed on SPSS version 23. Chi square and T test were used to check significant relation of risk factors with meconium aspiration syndrome. P value of <0.05 was consider significant. Results : Among total enrolled 150 babies, the mean age of babies was 37.37±18.96 hours, the male to female ratio of the babies was 1.03:1. The mean maternal age was 28.85±6.602 years. The thick meconium was observed in 89(59.33%) patients, 79(52.7%) patients went through vaginal delivery, postdate pregnancy was noted in 68(45.3%) patients, IUGR was observed in 52(34.7%) patients and poor APGAR score at 1 min was noted in 79(52.7%) patients. Conclusion: The observations of our study revealed that Meconium aspiration syndrome has significant relation


INTRODUCTION:
Significant morbidity and mortality has been observed with meconium aspiration syndrome [1-3]. One of the major causes of respiratory distress in term infants is meconium aspiration syndrome having incidence of 1.5 per 1000 live births [2]. The passage of meconium in-utero predisposes an infant to meconium aspiration and has been observed in 10-20% of term deliveries and incidence is even higher in post-term infants2'3' 6 [2-4]. Incidence of Meconium aspiration syndrome is lesser in pre-term deliveries. Recent literature showing reduction in mortality rate which is less than 15% appears to be associated with reduction in meconium aspiration syndrome owing to changes in obstetrical practices [2]. The risk of meconium aspiration syndrome in an infant born with meconium stained amniotic fluid and presence of meconium in airways at delivery. From these risk factors asphyxia is the most important risk factor [2,5].
Clinical features of meconium aspiration syndrome include respiratory distress within two hours of birth in an infant with meconium stained amniotic fluid, tachypnea and cyanosis. Chest auscultation shows inspiratory crepitations [6,7]. Chest X-rays reveals variable atelectasis and patchy opacifications with areas of hyperinflation. Blood gases show hypoxemia often accompanied by hypercarbia [2].
Complications of meconium aspiration syndrome include pneumonia, persistent pulmonary hypertension, interstitial emphysema and pneumothorax [8]. 15-30% cases show pneumothorax'. There is a difference in outcome in patients with meconium aspiration syndrome in developing countries and those in developed countries and factors associated with poor outcome in developing countries include poor monitoring during labour and low APGAR scores'. 9 There is limitation in assessing the outcome of meconium aspiration syndrome in developing countries these limitations are due to limited resources [1].
As the incidence of meconium aspiration syndrome in developing countries is high and no significant and local data is available to assess various risk factors for meconium aspiration syndrome so the rationale of my study is to study the frequency of various risk factors for meconium aspiration in neonatal population so that a liaison can be made between obstetricians and paediatricians to reduce the incidence of meconium aspiration syndrome.

MATERIALS AND METHODS:
A total number of 150 new-borns presenting to Department of Paediatric Medicine Sir Ganga Ram hospital with complaint of meconium staining was evaluated for the study. After explaining risks / benefits of the study and informed consent from parents, they were assessed by history and clinical examination. Sample size of 150 cases was calculated with 95% confidence level, 7% margin of error and taking expected percentage of low APGAR score at 5mins i.e. 20% (least among all) risk factor associated with meconium aspiration. Aspiration of meconium stained amniotic fluid before, during or after delivery resulting in respiratory distress assessed with respiratory rate more than 60/mm, vocal cord staining seen by direct laryngoscopy, cord blood pH 7.16 and X-ray chest showing opacifications was labelled as meconium aspiration syndrome.
Intra-uterine Growth Retardation was labelled when birth weight less than 2.5SD of the normal as per WHO growth charts. Thick Meconium was judged by the colour and texture of the meconium Green to dark-green and granular on visual inspection. The new-borns with Meconium Stained body presenting in first 72 hours of life either gender included in the study. The new-borns who had been associated congenital heart diseases assessed clinically with the help of auscultation, diaphragmatic hernia or any dysmorphism, was excluded. New-born not presenting with antenatal record were excluded from the study. ABG and X-ray Chest was done in all neonates.
The data collection tool (Annexure-I hereby attached) was applied to finally selected patients to collect information regarding risk factors like, Postterm pregnancy, IUGR, thick meconium, low APGAR score at 1 and 5 minutes as per operational definition. Data was entered and analysed by using computer program SPSS-10. Descriptive statistics was applied to calculate mean and standard deviation for maternal age.
Frequencies and percentages was calculated for risk factors like Post-term pregnancy, IUGR, thick meconium, low APGAR score at 1 and 5 minutes. Data was stratified for maternal age, mode of delivery (c/section/vaginal) to deal with effect modifiers. Poststratification, chi-square test was applied and p-value≤0.05 was considered significant.

RESULTS:
Among total 150 patients enrolled in this study the mean age of the babies was 37.37±18.96 hours with minimum and maximum ages of 6 & 72 hours respectively. In our study 76(50.67%) babies were male and 74(49.33%) babies were females. The male to female ratio of the babies was 1.03:1. The study results showed that the mean value maternal age was 28.85±6.602 years with minimum and maximum ages of 18 & 40 years respectively (Table-1). In our study statistically insignificant association was found between meconium aspiration syndrome and poor APGAR score at 1, mode of delivery i.e. vaginal or caesarean section and baby gender i.e. male or female with P value 0.734, 0.821 and 0.892 respectively.
In this study statistically significant association was found between meconium aspiration syndrome and poor APGAR score at 5 minutes, maternal age , postdated pregnancy, IUGR, and thick meconium with P value 0.034, 0.000, 0.001, 0,021 and 0.000 respectively.

DISCUSSION:
This present descriptive cross sectional study was conducted at Pediatrics Unit of Sir Ganga ram Hospital Lahore to study the frequency of various factor leading to MAS.MAS is a common cause of lung disease in neonates. Cause of MAS is inhalation of thick meconium. Most of time it is due to hypoxia of fetus which can increased the peristaltic movement, gasping reflux and relaxation of anal sphincters [10]. Meconium passage occurs in up to 20% of full term gestations and can occur in more than 35% of pregnancies continuing beyond 42 weeks' gestation [11][12][13][14][15][16].
In our study 79(52.7%) patients went through vaginal delivery and 71(47.3%) patients went through CS, the postdate pregnancy was noted in 68(45.3%) patients, the IUGR was observed in 52(34.7%) patients, the poor APGAR score at 1 min was noted in 79(52.7%) patients and poor APGAR score at 5 min was noted in 69(46%) patients. In this study the thick meconium was observed in 89(59.33%) patients. Maternal age and mode of delivery showed insignificant difference with factors. Incidence of MAS occurs in long time period due to less cases of post term deliveries, close monitoring and management of fetal heart rate and reduction in the low APGAR score births [17].
At 41 weeks or beyond these weeks elective induction of labour found to be highly associated with low rate of MAS and minimal perinatal mortality and morbidity when compared with expectant management [18].
A study by UzmaFirdauset al [19] reported 9.8% incidence of MSAF and 1.8% of MAS. He reported that fetal respiratory distress and low APGAR score are the main risk factors of MAS, maternal risk factors are not prominent.In the study by swain et al, the incidence of MSAF was 13.97% and that of MAS was 8.57% [20] In the study by Manganaro et al no significant difference in parity, maternal age, gestational age, metabolic acidemia, sex, low APGAR score at 1 minute and 5 minute and need for endotracheal intubation was observed between both groups MSAF and non-MSAF infants [21].
Bhat RY et al [22] however found thick meconium as the only significant factor contributing to MAS. Another work by Khazardoost et al [23] did not find the role any maternal factors in predicting the progression to MAS. In a previous study conducted by Usta et al study [24] it was reported late maturity not a risk factor of MAS. Conclusion of his study favors the concept that incidence of meconium aspiration occurs in normally mature infants that leads the infants fetal compromise and MAS. Some studies demonstrated that the presence of fetal compromise like low Apgar score, [25][26][27] abnormal heart rhythm [28][29] and cesarean deliveries enhance the chances of MAS in infantsand of MAS in the meconium-stained infant [30].

CONCLUSION:
The observations of our study revealed that Meconium aspiration syndrome has significant relation with maternal age (P value 0.000), postdated pregnancy(P Value 0.001), IUGR (P value 0.021), poor APGAR score at 5 minutes(P value 0.034), and thick meconium (P value 0.000).