The Role of Tacrolimus and Sirolimus in Modulation Humeral Immunity in Male Albino Rats

Abstract

The object of this study  ware to modulated the effect of Tacrolimus and Sirolimus in Humeral immunity epically on titration of antibody and interlukin-2  in albino male rats. Fiftieth adult albino male  rats were housed and arbitrarily divided into five equal groups (ten rats/ group)and administered as follows for 90 days :control group received distilled water ,groups  T1 received sirolimus therapeutics dose 0.02mg/ kg B.W and T2:received sirolimus  two fold dose  0.04mg/ kg BW and T3: received tacrolimus  therapeutic dose 0.05mg/ kg BW and T4:received tacrolimus two fold dose 0.1mg/ mg B W.blood sample ware collect from each group in 30,60,90 days of experimental .Passive  hemagglutination  test used to assessment titrations of antibody, direct  Enzyme Linked Immune Sorbent Assay  used to assessment titrations of interlukin-2.The results at 30 days the titration of interleukin-2 ware decreased in all treated groups as a camper with control group and the decreasing depended on the dose and the time of exposure. The more significant in T2 and T4 in .30,60,90 days of experimental and the Tacrolimus showed more significant in reduction of interlukin-2 titrations  than Sirolimus while  the titration of antibody also show decreased in all treated groups T1,T2,T3,and T4 showed significant (p<0.05) in 30,60,90 days in comparison with the control one. Also the all treated group showed more decreased in 60,90 day and that more clear than in 30 day of experimental .The sirolimus more significant  on the titrations of antibody than tacrolimus.

Keywords:Tacrolimus, Sirolimus, interlukin-2, Antibody.