Identification of Lubricating Oil-Degrading Microorganisms in Oil Polluted Soils from Five Auto- mechanic Workshops in Accra, Ghana

Abstract

Trichothecium, Trichoderma, Aspergillus niger, Fusarium, and Penicillium spp. from oil contaminated soil from 5 Mechanic Shops in Accra, Ghana showed zones of clearance of oil on Minimum Salt Enrichment Medium (MSEM) Agar seeded with 1000ppm Engen™ Lubricating Oil (ELO), so were counted as presumptive lubricating oil-utilizing moulds. Significant increases (P ? 0.05) in viable counts, fungal dry weights and optical densities; significant decreases (P ? 0.05) in pH’s of pure cultures of the moulds in MSEM+1.0%(v/v) ELO medium at 30°C for 0 - 25 day’s; positive correlations between viable counts and fungal dry weights, viable counts and optical densities, and fungal dry weights and optical densities; and negative correlations between pH and viable counts, and pH and optical densities, confirmed the moulds as lubricating oil consuming fungi with potential for use in bioremediation of oil polluted soils. Aspergillus niger exhibited the highest bioremediation capacity and Trichothecium the least.

Keywords: Lubricating Oil, Pollution, Fungi, Bioremediation