Chemistry of Caffeine in Coffee and Its Determination Using UV/Vis Spectrophotometer: A Review Article

Abstract

This review is all about the chemistry of caffeine and some methods applied to determine the concentration of caffeine in coffee. Coffee is the major sources of caffeine. In pure form, caffeine is a crystalline white powder moderately soluble in water and in a wide range of organic solvent such as ethanol, ethyl acetate, methanol, benzene etc. When it is removed from the source plants and reduced to its most natural state, it forms a white powder. Consequently, the ingestion of caffeine, an important ingredient of coffee as well as tea, has become a daily habit for many persons. Among different methods used to determine the concentration of caffeine in coffee, spectrophotometric method is an effective method used for the determination of caffeine in Coffee raw. The method was found to be fast, simple, cost effective and environmental friendly for the determination of caffeine in aqueous solution of coffee with satisfactory results.

Keywords: Coffee, caffeine, UV-Viscible spectrophotometer