Studies on Urinary Tract Infection among Diabetics in Some Eastern States of Nigeria

Abstract

This study evaluated the incidence of Urinary tract infection (UTI) among diabetics in some Eastern States of Nigeria. Three hundred (300) midstream urine samples from diabetic patients with fasting blood sugar level between150mg/dl to 450mg/dl (8.3mmol/l to 25mmol/l), 150 males and 150 females) and 200 non diabetic subjects (100 each male and female) were cultured unto freshly prepared Eosine methylene blue (EMB) oxoid, MacConkey (Difco) and blood agar plates and incubated for 24hours at 37^oc. Urinalysis using Combi-9 urine test strip and microscopic examination of urine deposits were done. Gram staining and some biochemical tests, antimicrobial sensitivity tests were carried out on isolates using agar diffusion methods. Seventy eight (26%) urine samples from diabetic patients showed growth of specific organism with 45(15%) in female patients and 33(11%) from male patients. Out of the 200 control urine samples 30(10%) showed growth of specific organism (15 each from male and female subjects). Escherichia coli was the predominant isolate 55(70.5%), Proteus mirabilis 10(12.5%), Klebsiella 8(10%) and Staphylococcus saprophyticus 5(7%). While in the control E.coli was isolated 20(66.7%), Proteus 10(33.3%). All organisms isolated were 100% resistant to ampicilline, nitrofurantoin and100% sensitive to gentamicin, nalidixic acid and ofloxacin. The result of this work showed no significant difference between positive nitrite urine samples 60(20%) and specific bacterial growth 26% (p>0.05). No qualitative and quantitative differences were observed in the distribution of microbial pathogens of UTI in diabetic and non-diabetic male and female patients studied.

Keywords: Urinary tract infections, diabetics, antibiotic sensitivity.