Hepatoprotective Effect of Royal Jelly Against Cisplatin-Induced Biochemical, Oxidative Stress, Anti-Oxidants And Histopathological Abnormalities

Abstract

The study aimed to evaluate the changes of liver functions, oxidative stress, anti-oxidants and histopathological investigations in Cisplatin-induced hepatotoxicity and the royal jelly ameliorative effects. The forty male mice were divided into 4 groups. The first group was control, Group II: animals were received royal jelly (150 mg/kg), Group III: animals were received cisplatin (3.5 mg/kg body weight of Cisplatin once every 2 days), group IV: animals were received the royal jelly + Cisplatin, for 10 days. At the end of experiment Blood was collected and analyzed for blood alanine aminotransferase (ALT), aspartate aminotransferase (AST), Albumin. The Liver samples were stored for the measurement of malondialdehyde (MDA), total antioxidant and catalase (CAT) activities and another hepatic tissues were processed for histopathology. Cisplatin given to mice induced a marked hepatic injuries, characterized with a significant increase in serum ALT, AST and MDA and lower albumin, total antioxidant, and CAT activities. In the groups that were administered royal jelly in association with cisplatin, improvement was observed in oxidative stress parameter (MDA), other hepatic biochemical parameters, and histopathological examinations with royal jelly being more effective. The results obtained suggested that royal jelly significantly attenuated the cisplatin-hepatotoxicity, because it act as free radical scavenger and lipid peroxidation inhibitor.

Keywords: Cisplatin; Royal jelly; Antioxidants; Hepatotoxicity